antigen sequence: RGLHELSPPS FEDDLARPAE VSKASPRAQP QQGIHRRTPY STCP, corresponding to amino acids 181-224 of Human TMEM221.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Membrane; Multi-pass membrane protein
- Putative transmembrane protein ENSP00000342162 antibody
- TMEM221 antibody
- Transmembrane protein 221 antibody
Immunocytochemistry/ Immunofluorescence - Anti-TMEM221 antibody (ab122731)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nuclear membrane. Recommended concentration of ab122731 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM221 antibody (ab122731)
ab122731 at 1/20 dilution staining TMEM221 in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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