antigen sequence: LTCRLASYEQ RRMFSEREHS LHHSHEMAEK EITDNMSNGG PQLIFNGRV, corresponding to C terminal amino acids 130-178 of Human TMEM196.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Immunocytochemistry/ Immunofluorescence - Anti-TMEM196 antibody (ab122839)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and golgi apparatus. Recommended concentration of ab122839 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM196 antibody (ab122839)
ab122839 at 1/50 dilution staining TMEM196 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"