antigen, corresponding to amino acids 13-65 (GKAVWERPLH GLWCCSGQED PKRWVGSSSP ISKEKLPNAE TEKFWMFYRF DAI) of Human TMEM186.
Human bronchus tissue; Transfected HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Belongs to the TMEM186 family.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-TMEM186 antibody (ab121565)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoskeleton (actin filaments) and cytoskeleton (microtubules). Recommended concentration of ab121565 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM186 antibody (ab121565)
ab121565 at 1/75 dilution staining TMEM186 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry.
Western blot - Anti-TMEM186 antibody (ab121565)
All lanes : Anti-TMEM186 antibody (ab121565) at 1/250 dilution Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : TMEM186 transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)) Developed using the ECL technique
has not yet been referenced specifically in any publications.
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