Anti-TMEM180抗体(ab108080)

概述

  • 产品名称Anti-TMEM180抗体
  • 描述
    兔多克隆抗体to TMEM180
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 361-411 (CLFIASNRVF TEGTCKLLTL VVTDLVDEDL VLNHRKQAAS ALLFGMVALV T) of Human TMEM180 (NP_079065).

  • 阳性对照
    • THP-1 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-TMEM180 antibody 图像

  • Anti-TMEM180 antibody (ab108080) at 1 µg/ml + THP-1 cell lysate at 10 µg

    Predicted band size : 57 kDa

    Gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-TMEM180 antibody (ab108080)参考文献

ab108080 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount 40 µg
Specification Skin Fibroblasts
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin fibroblasts)
Specification Skin fibroblasts
Fixative Methanol
Permeabilization Yes - 0.1% triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Dec 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"