antigen sequence: LITSGGAAAA MSSPPQYYTI YPQDNSAFVV DEGCLSFTDG GNHRPNPDVD QLEETQLEEE ACACFSPPPY EEIYSLPR, corresponding to C terminal amino acids 166-243 of the Human TMEM174 protein (UniProt ID: Q8WUU8).
Human kidney tissue; RT-4 and U-251 MG cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
Transmembrane protein 174 antibody
Immunocytochemistry/ Immunofluorescence - Anti-TMEM174 antibody (ab122429)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122429 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM174 antibody (ab122429)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TMEM174 with ab122429. Staining shows strong membranous positivity in cells in tubules.
Western blot - Anti-TMEM174 antibody (ab122429)
All lanes : Anti-TMEM174 antibody (ab122429) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma lysate Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate
has not yet been referenced specifically in any publications.
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