概述

  • 产品名称Anti-TMEM173抗体
    参阅全部 TMEM173 一抗
  • 描述
    兔多克隆抗体to TMEM173
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Chimpanzee, Rhesus monkey
    预测可用于: Dog
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 310 - 360 of Human TMEM173 (NP_938023).

  • 阳性对照
    • Human breast cancer tissue

性能

应用

Our Abpromise guarantee covers the use of ab92605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. PubMed: 21838860
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the TMEM173 family.
  • 翻译后修饰Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
  • 细胞定位Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt
  • 数据库链接
  • 别名
    • endoplasmic reticulum IFN stimulator antibody
    • Endoplasmic reticulum interferon stimulator antibody
    • ERIS antibody
    • FLJ38577 antibody
    • hMITA antibody
    • hSTING antibody
    • Mediator of IRF3 activation antibody
    • MITA antibody
    • Mitochondrial mediator of IRF3 activation antibody
    • MPYS antibody
    • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
    • NET23 antibody
    • Stimulator of interferon genes antibody
    • Stimulator of interferon genes protein antibody
    • STING antibody
    • TM173_HUMAN antibody
    • Tmem173 antibody
    • Transmembrane protein 173 antibody
    see all

Anti-TMEM173 antibody 图像

  • All lanes : Anti-TMEM173 antibody (ab92605) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemistry analysis of TMEM173 expression in formalin-fixed, paraffin-embedded Human breast cancer tissue an isotype control (1) or ab92605 at 5µg/ml (2 and 3).
  • ICC/IF image of ab92605 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92605, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TMEM173 antibody (ab92605)参考文献

This product has been referenced in:
  • Panchanathan R  et al. Identification of a negative feedback loop between cyclic di-GMP-induced levels of IFI16 and p202 cytosolic DNA sensors and STING. Innate Immun N/A:N/A (2013). Read more (PubMed: 24131791) »
  • Orzalli MH  et al. Nuclear interferon-inducible protein 16 promotes silencing of herpesviral and transfected DNA. Proc Natl Acad Sci U S A 110:E4492-501 (2013). WB . Read more (PubMed: 24198334) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hep G2)
Specification Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"