Anti-TMEM16B抗体(ab113443)

概述

  • 产品名称
    Anti-TMEM16B抗体
    参阅全部 TMEM16B 一抗
  • 描述
    兔多克隆抗体to TMEM16B
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide from the N terminal region of Human TMEM16B, conjugated to KLH.

  • 阳性对照
    • Human small intestine and spleen tissues; Rat brain tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab113443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 114 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能
    Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
  • 组织特异性
    Retina, especially in the photoreceptor synaptic terminals.
  • 序列相似性
    Belongs to the anoctamin family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ano2 antibody
    • ANO2_HUMAN antibody
    • Anoctamin 2 antibody
    • Anoctamin-2 antibody
    • C12orf3 antibody
    • Chromosome 12 open reading frame 3 antibody
    • DKFZp434P102 antibody
    • Transmembrane protein 16B (eight membrane-spanning domains) antibody
    • Transmembrane protein 16B antibody
    see all

图片

  • ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.
  • ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.
  • Lane 1 : Anti-TMEM16B antibody (ab113443) at 1 µg/ml
    Lane 2 : Anti-TMEM16B antibody (ab113443) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate


    Predicted band size : 114 kDa

文献

ab113443 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PBS/HCl/Borax
Permeabilization
Yes - 0.2% PBS-Tx
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon Ca)
Specification
Colon Ca
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Zytomed Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

提交于 Oct 17 2012

The laboratory that produces ab113443 has sent the alignment of the immunogen with the rat and mouse proteins. As I mentioned in my previous e-mail, the identity of the immunogen sequence is considered proprietary. Homology between human and mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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