Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human TMEM16A.
(Peptide available as
This antibody gave a positive signal in Human Brain membrane tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
Acts as a calcium-activated chloride channel. Required for normal tracheal development.
Broadly expressed with higher levels in liver and skeletal muscle.
Belongs to the anoctamin family.
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
Cell membrane. Cytoplasm.
Information by UniProt
ANO 1 antibody
Western blot - Anti-TMEM16A antibody (ab84915)
Anti-TMEM16A antibody (ab84915) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
28 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-TMEM16A antibody (ab84915)
ICC/IF image of ab84915 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84915, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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