概述

  • 产品名称Anti-TMEM16A抗体
    参阅全部 TMEM16A 一抗
  • 描述
    兔多克隆抗体to TMEM16A
  • 特异性This antibody reacts specifically with human 114 kDa TMEM16A protein.
  • 经测试应用适用于: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human TMEM16A aa 700-800 (internal sequence).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 114 kDa.
ICC/IF 1/1000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20686072
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Acts as a calcium-activated chloride channel. Required for normal tracheal development.
  • 组织特异性Broadly expressed with higher levels in liver and skeletal muscle.
  • 序列相似性Belongs to the anoctamin family.
  • 结构域The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
  • 细胞定位Cell membrane. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANO 1 antibody
    • ANO1 antibody
    • ANO1_HUMAN antibody
    • Anoctamin 1 antibody
    • Anoctamin 1 calcium activated chloride channel antibody
    • Anoctamin-1 antibody
    • Anoctamin1 antibody
    • Ca2+ activated Cl- channel antibody
    • Calcium Activated Chloride Channel antibody
    • Discovered on gastrointestinal stromal tumors protein 1 antibody
    • DOG 1 antibody
    • FLJ10261 antibody
    • Membrane protein antibody
    • Oral cancer overexpressed 2 antibody
    • Oral cancer overexpressed protein 2 antibody
    • ORAOV 2 antibody
    • ORAOV2 antibody
    • TAOS 2 antibody
    • TAOS2 antibody
    • TMEM 16A antibody
    • TMEM16A antibody
    • Transmembrane protein 16A (eight membrane spanning domains) antibody
    • Transmembrane protein 16A antibody
    • Tumor amplified and overexpressed sequence 2 antibody
    • Tumor-amplified and overexpressed sequence 2 antibody
    see all

Anti-TMEM16A antibody 图像

  • ICC/IF image of ab72984 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72984, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TMEM16A antibody (ab72984)参考文献

This product has been referenced in:
  • Wang M  et al. Downregulation of TMEM16A calcium-activated chloride channel contributes to cerebrovascular remodeling during hypertension by promoting basilar smooth muscle cell proliferation. Circulation 125:697-707 (2012). WB ; Rat . Read more (PubMed: 22215857) »
  • Davis AJ  et al. Expression profile and protein translation of TMEM16A in murine smooth muscle. Am J Physiol Cell Physiol 299:C948-59 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20686072) »

See all 2 Publications for this product

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Thank you for your reply and for kindly providing the word document. This has made conducting the alignments much more straight forward.

I am sorry to confirm that as far as we are aware,none of our TMEM16A antibodieshas been tested with sam...

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Application Western blot
Sample Rat Tissue lysate - whole (arterial homogenate)
Loading amount 50 µg
Specification arterial homogenate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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提交于 Sep 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"