antigen sequence corresponding to amino acids 32-79 (KVGSVQSPSA GFFFSELAGL DASQPSRNPK EHLSSPVYIW DLARYYAN) of Human TMEM14E.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the UPF0136 (TMEM14) family.
Information by UniProt
- TM14E_HUMAN antibody
- TMEM14E antibody
- Transmembrane protein 14E antibody
Immunocytochemistry/ Immunofluorescence - Anti-TMEM14E antibody (ab121437)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and vesicles. Recommended concentration of ab121437 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM14E antibody (ab121437)
ab121437, at 1/400 dilution, staining TMEM14E in paraffin-embedded Human appendix tissue, by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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