ICC/IF, WB, IHC-Pmore details
antigen: THRIYKQKLE ELAALQTLCS SSISKQKKHL KDLKLTLQRC KRHASREEAE LVQQMAANIK ERQDVFFDME AYLPKKNGLY LNLVLGNVNV TLLSNQAKFA YKD, corresponding to amino acids 25-127 of Human TMEM120B (A0PK00).
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- 纯度Immunogen affinity purified
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/100 - 1/250. Predicted molecular weight: 40 kDa.
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- 序列相似性Belongs to the TMEM120 family.
Information by UniProt
- T120B_HUMAN antibody
- TMEM120B antibody
- Transmembrane protein 120B antibody
Anti-TMEM120B antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-TMEM120B antibody (ab121413)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121413 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-TMEM120B antibody (ab121413)
Lane 1 : Anti-TMEM120B antibody (ab121413) at 1/100 dilution
Lane 2 : Anti-TMEM120B antibody (ab121413) at 100
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1kDa) in HEK293T cells.
Predicted band size : 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM120B antibody (ab121413)
Immunohistochemical staining of Human stomach tissue shows strong cytoplasmic positivity in glandular cells, using 1/20 ab121413.
Anti-TMEM120B antibody (ab121413)参考文献
has not yet been referenced specifically in any publications.
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