Anti-TMEM119抗体- C-terminal (ab185333)

概述

  • 产品名称
    Anti-TMEM119抗体- C-terminal
    参阅全部 TMEM119 一抗
  • 描述
    兔多克隆抗体to TMEM119 - C-terminal
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human TMEM119 aa 194-282 (C terminal).
    Sequence:

    GDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVLEGA VVAGEGQGELEGSLLLAQEAQGPVGPPESPCACSSVHPS


    Database link: Q4V9L6

  • 阳性对照
    • IHC-P: Human tonsil tissue. IF: Human cortical tissue.
  • 常规说明

    This antibody is suitable for detecting human TMEM119 by IHC. To detect mouse TMEM119 by IHC, we recommend using ab209064.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab185333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 26884166

靶标

  • 细胞定位
    Membrane; Single-pass type I membrane protein
  • 数据库链接
  • 别名
    • OBIF antibody
    • Osteoblast induction factor antibody
    • PSEC0199 antibody
    • Transmembrane protein 119 antibody
    • UNQ731/PRO1415 antibody
    see all

图片

  • Ab185333 staining TMEM119 in Human Brain tissue (acute surgical samples & post-mortem) sections by Immunohistochemistry (IHC-Fr-Frozen sections). Tissue was fixed with formaldehyde and permeabilized with 0.3-0.5% Triton X in PBS and blocked with 10% Serum for 1 hours at 23°C. Samples were incubated with primary antibody at (1:100 dilution in 1% serum in PBS-Triton X) for 16 hours at 4°C. An Alexa Fluor® 488 Goat anti-rabbit (4µg/ml) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded human tonsil labeling TMEM119 with ab185333 at 1/500 dilution.

文献

This product has been referenced in:

See all 2 Publications for this product

客户评价及客户问答

Application
Flow Cytometry
Sample
Human Cell (Human immortalized microglial cell line)
Permeabilization
Yes - 1X Permeabilization buffer (eBioscience 00-8333-56)
Gating Strategy
A discrete population on the FSC-SSC scatter plot
Specification
Human immortalized microglial cell line
Preparation
Cell harvesting/tissue preparation method: Scraped cells from the plate
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 01 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain Tissue acute surgical samples & post-mortem)
Permeabilization
Yes - 0.3-0.5% Triton X in PBS
Specification
Brain Tissue acute surgical samples & post-mortem
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph6.0 Citrate Buffer
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Mr. Avinash Hari Narayanan

Verified customer

提交于 Aug 11 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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