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This antibody is suitable for detecting human TMEM119 by IHC. To detect mouse TMEM119 by IHC, we recommend using ab209064.
Our Abpromise guarantee covers the use of ab185333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 26884166|
Ab185333 staining TMEM119 in Human Brain tissue (acute surgical samples & post-mortem) sections by Immunohistochemistry (IHC-Fr-Frozen sections). Tissue was fixed with formaldehyde and permeabilized with 0.3-0.5% Triton X in PBS and blocked with 10% Serum for 1 hours at 23°C. Samples were incubated with primary antibody at (1:100 dilution in 1% serum in PBS-Triton X) for 16 hours at 4°C. An Alexa Fluor® 488 Goat anti-rabbit (4µg/ml) was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil labeling TMEM119 with ab185333 at 1/500 dilution.
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