使用敲除细胞株进行验证
重组
RabMAb

Anti-TMEM119抗体[106-6] - Microglial marker (ab210405)

概述

  • 产品名称
    Anti-TMEM119抗体[106-6] - Microglial marker
    参阅全部 TMEM119 一抗
  • 描述
    兔单克隆抗体[106-6] to TMEM119 - Microglial marker
  • 特异性
    Human reactivity has not been tested.
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
    Database link: Q8R138

  • 阳性对照
    • Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged TMEM119
  • 常规说明

    Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

    Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.

    The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    106-6
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab210405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 0.1 - 0.5 µg/ml.

 

靶标

  • 细胞定位
    Membrane; Single-pass type I membrane protein
  • 数据库链接
  • 别名
    • OBIF antibody
    • Osteoblast induction factor antibody
    • PSEC0199 antibody
    • Transmembrane protein 119 antibody
    • UNQ731/PRO1415 antibody
    see all

图片

  • Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

    No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

    The data was provided by Ben Barres’ lab (Stanford University).

  • Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate 0,01M
Permeabilization
Yes - PBS 0,1M triton 0,1%
Specification
spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Temporal Cortex)
Antigen retrieval step
None
Permeabilization
No
Specification
Temporal Cortex
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formalin
Username

Mrs. Julie Castro

Verified customer

提交于 Jul 10 2017

Application
Flow Cytometry
Sample
Rat Cell (Cortex)
Permeabilization
No
Gating Strategy
CD11b+ Microglia
Specification
Cortex
Preparation
Cell harvesting/tissue preparation method: Microglia were isolated by CD11b magnetic bead isolation from the cortex of adult, female SD rats.
Sample buffer: PBS/0.5% BSA
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 25 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Brain, microglia)
Permeabilization
Yes - 0.3% Triton-X in PBS
Specification
Brain, microglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 05 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Brain
Blocking step
CytoQ Immunodiluent & Block as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Sk De

Verified customer

提交于 Mar 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册