Anti-TMEM119抗体[106-6] - Microglial marker (ab210405)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
-
产品名称Anti-TMEM119抗体[106-6] - Microglial marker
参阅全部 TMEM119 一抗 -
描述兔单克隆抗体[106-6] to TMEM119 - Microglial marker
-
宿主Rabbit
-
特异性Human reactivity has not been tested.
-
经测试应用适用于: Flow Cytmore details
-
种属反应性与反应: Mouse
-
免疫原
Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact sequence is proprietary.
Database link: Q8R138 -
阳性对照
- Flow Cytometry: Acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells), HEK-293T transfected with Myc-His tagged TMEM119
-
常规说明
Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author’s permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.
Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend ab209064.
The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
-
克隆单克隆
-
克隆编号106-6
-
同种型IgG
相关产品
-
Alternative Products
-
Alternative Versions
应用
Our Abpromise guarantee covers the use of ab210405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use a concentration of 0.1 - 0.5 µg/ml.
|
靶标
-
细胞定位Membrane; Single-pass type I membrane protein
-
数据库链接
- Entrez Gene: 231633 Mouse
- SwissProt: Q8R138 Mouse
-
别名
- OBIF antibody
- Osteoblast induction factor antibody
- PSEC0199 antibody
see all
图片
-
![Flow Cytometry - Anti-TMEM119 antibody [106-6] (ab210405)](http://www.abcam.cn/ps/products/210/ab210405/Images/ab210405-272564-160823MarikoLayout2withwatermarkanddssized.jpg)
Flow Cytometry - Anti-TMEM119 antibody [106-6] (ab210405)Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).
The data was provided by Ben Barres’ lab (Stanford University).
-
![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)](http://www.abcam.cn/ps/products/210/ab210405/Images/ab210405-297063-210405FC1.jpg)
Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.
文献
This product has been referenced in:
- Bennett ML et al. New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113:E1738-46 (2016). Read more (PubMed: 26884166) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"