Anti-TMEM109抗体(ab121349)

概述

  • 产品名称Anti-TMEM109抗体
  • 描述
    兔多克隆抗体to TMEM109
  • 经测试应用适用于: IHC-Fr, ICC, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    antigen sequence: LEAKVRGLER QVEELRWRQR RAAKGARSVE E, corresponding to amino acids 212-242 of Human TMEM109.

  • 阳性对照
    • Human Pancreas tissue; U 251 MG, RT4 lysate, Human Liver and Tonsil lysates; U 251 MG cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. using Fixation/Permeabilization: PFA/Triton X-100.

靶标

Anti-TMEM109 antibody 图像

  • ab121349, at 1/1000, staining TMEM109 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
    Note: strong cytoplasmic positivity in exocrine glandular cells.
  • ab121349 at 4 µg/ml staining TMEM109 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
    Note: positivity in nucleus but not nucleoli, nuclear membrane and cytoplasm.
  • All lanes : Anti-TMEM109 antibody (ab121349) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-TMEM109 antibody (ab121349)参考文献

ab121349 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate)
Loading amount 25 µg
Specification Prostate
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate cell line LNCaP)
Specification Prostate cell line LNCaP
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

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The antibody has been tested in WB, ICC/IF and IHC-P with human samples. This antibody is fully guaranteed to be used in these listed applications and species.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"