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A 14-22 amino acid region within synthetic peptide: KAGDPVILYV NKVGPYHNPQ ETYHYYQLPV CCPEKIRHKS LSLGEVLDGD, corresponding to N terminal amino acids 37-86 of Human TM9SF1
Our Abpromise guarantee covers the use of ab62222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:312500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TM9SF1 with ab62222 at 4-8µg/ml. Arrow indicates positively labelled epithelial cells of the renal tubule. Magnification: 400X.
ab62222 has not yet been referenced specifically in any publications.