概述
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产品名称Anti-TM4SF3抗体
参阅全部 TM4SF3 一抗 -
描述兔多克隆抗体to TM4SF3
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宿主Rabbit
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特异性Detects endogenous levels of total TM4SF3 protein.
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经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide derived from an internal region of human TM4SF3
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阳性对照
- 293 cell extract COLO cell extract HeLa cells
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab70007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). | |
| ELISA | 1/20000. | |
| ICC/IF | 1/500 - 1/1000. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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组织特异性Gastric, colon, rectal, and pancreatic carcinomas.
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序列相似性Belongs to the tetraspanin (TM4SF) family.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 7103 Human
- Omim: 600769 Human
- SwissProt: P19075 Human
- Unigene: 170563 Human
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别名
- anti TSPAN8 antibody
- CO029 antibody
- Tetraspanin 8 antibody
see all
图片
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Western blot - Anti-TM4SF3 antibody (ab70007)All lanes : Anti-TM4SF3 antibody (ab70007) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : COLO cell extract
Lane 3 : COLO cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TM4SF3 antibody (ab70007)Immunofluorescence of HeLa cells treated with ab70007 at 1/500 dilution. Right panel was treated with immunising peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM4SF3 antibody (ab70007)IHC image of ab70007 staining in human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
文献
ab70007 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"