Anti-TM4SF3抗体(ab70007)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TM4SF3
  • 特异性
    Detects endogenous levels of total TM4SF3 protein.
  • 经测试应用
    适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal region of human TM4SF3

  • 阳性对照
    • 293 cell extract COLO cell extract HeLa cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性
    Gastric, colon, rectal, and pancreatic carcinomas.
  • 序列相似性
    Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • anti TSPAN8 antibody
    • CO029 antibody
    • Tetraspanin 8 antibody
    • Tetraspanin-8 antibody
    • TM4SF3 antibody
    • Transmembrane 4 superfamily member 3 antibody
    • TSN8_HUMAN antibody
    • Tspan 8 antibody
    • Tspan-8 antibody
    • TSPAN8 antibody
    • TSPAN8 antibody antibody
    • Tumor associated antigen CO 029 antibody
    • Tumor-associated antigen CO-029 antibody
    see all

图片

  • All lanes : Anti-TM4SF3 antibody (ab70007) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : COLO cell extract
    Lane 3 : COLO cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunofluorescence of HeLa cells treated with ab70007 at 1/500 dilution. Right panel was treated with immunising peptide.
  • IHC image of ab70007 staining in human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

ab70007 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam regarding ab70007.


The amino acid range of this immunogen is proprietary. This antibody reacts with the second extracellular domain of the protein. We have not tested for cross reactivity with other tetras...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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