Synthetic peptide corresponding to a region within internal sequence amino acids 155-185 of Human TM222, conjugated to KLH.
Mouse bladder tissue lysates; Human bladder carcinoma tissue
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103689 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 23 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The function of this protein has not been specifically defined.
Membrane; Multi-pass membrane protein
Western blot - Anti-TM222 antibody (ab103689)
Anti-TM222 antibody (ab103689) at 1/100 dilution + Mouse bladder tissue lysates at 35 µg
Predicted band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM222 antibody (ab103689)
ab103689, at a 1/50 dilution, staining TM222 in formalin fixed, paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry, followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"