Anti-TLR9抗体(ab88101)

概述

  • 产品名称Anti-TLR9抗体
    参阅全部 TLR9 一抗
  • 描述
    小鼠单克隆抗体to TLR9
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 99-215 of human TLR9 (AAH32713) with a proprietary tag.

  • 阳性对照
    • WB: IMR-32 cell lysate ICC/IF: HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab88101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 细胞定位Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody 图像

  • Anti-TLR9 antibody (ab88101) at 1 µg/ml + IMR-32 cell lysate at 50 µg

    Predicted band size : 115 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
  • ab88101, at 10 µg/ml, staining TLR9 in HeLa cell by Immunofluorescence.
  • Detection limit for ab88101 is approximately 0.1 ng/ml as a capture antibody.

Anti-TLR9 antibody (ab88101)参考文献

ab88101 has not yet been referenced specifically in any publications.

Product Wall


I discovered that the immunogen for ab88101 is actually a recombinant protein rather than a peptide. Thus, the blocking study that I suggested is not applicable. However, I've sent you a positive control Jurkat whole cell lysate (http://www.abcam....

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