Anti-TLR9抗体(ab62577)

概述

  • 产品名称Anti-TLR9抗体
    参阅全部 TLR9 一抗
  • 描述
    兔多克隆抗体to TLR9
  • 经测试应用适用于: ICC, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 15 amino acids near the center of human TLR9

  • 阳性对照
    • Jurkat cells and cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab62577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 115 kDa).
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 细胞定位Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 289 antibody
    • CD289 antibody
    • TLR 9 antibody
    • TLR9 antibody
    • TLR9_HUMAN antibody
    • Toll like receptor 9 antibody
    • Toll like receptor 9 isoform A precursor antibody
    • Toll like receptor 9 isoform B antibody
    • Toll-like receptor 9 antibody
    see all

Anti-TLR9 antibody 图像

  • ab62577 at 2µg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.
  • Lane 1 : Anti-TLR9 antibody (ab62577) at 0.5 µg/ml
    Lane 2 : Anti-TLR9 antibody (ab62577) at 1 µg/ml
    Lane 3 : Anti-TLR9 antibody (ab62577) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 115 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of TLR9 in Mouse Spleen cells using ab62577 at 10 ug/ml.

Anti-TLR9 antibody (ab62577)参考文献

ab62577 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62577.
To check the status of the order please contact our Cus...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"