Anti-TLR9抗体(ab62577)
Key features and details
- Rabbit polyclonal to TLR9
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TLR9抗体
参阅全部 TLR9 一抗 -
描述
兔多克隆抗体to TLR9 -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to 15 amino acids near the center of human TLR9
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阳性对照
- Jurkat cells and cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62577于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 2 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 115 kDa).
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说明 |
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ICC
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 115 kDa). |
靶标
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功能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
组织特异性
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
序列相似性
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
细胞定位
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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数据库链接
- Entrez Gene: 54106 Human
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- Unigene: 87968 Human
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别名
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
图片
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Lane 1 : Anti-TLR9 antibody (ab62577) at 0.5 µg/ml
Lane 2 : Anti-TLR9 antibody (ab62577) at 1 µg/ml
Lane 3 : Anti-TLR9 antibody (ab62577) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 115 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
ab62577 at 2µg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62577 被引用在 1 文献中.
- Tripathi A et al. Heteromerization of chemokine (C-X-C motif) receptor 4 with a1A/B-adrenergic receptors controls a1-adrenergic receptor function. Proc Natl Acad Sci U S A 112:E1659-68 (2015). PubMed: 25775528