Customer reviews and FAQs

概述

  • 产品名称
    Anti-TLR9抗体
    参阅全部 TLR9 一抗
  • 描述
    兔多克隆抗体to TLR9
  • 经测试应用
    适用于: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to 16 amino acids near to the C terminus of the protein (Human).

  • 阳性对照
    • Mouse spleen cells and cell lysate .

性能

应用

Our Abpromise guarantee covers the use of ab37154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21549006
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性
    Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 26 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 细胞定位
    Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    Anti-TLR9 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (ab37154)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (ab37154)
      Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling TLR9 with Anti-TLR9 antibody (ab37154) at 5μg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TLR9 antibody (ab37154)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TLR9 antibody (ab37154)
      ab37154 at 2 µg/ml staining TLR9 in mouse spleen cells by IHC.
    • Immunocytochemistry/ Immunofluorescence-Anti-TLR9 antibody(ab37154)
      Immunocytochemistry/ Immunofluorescence-Anti-TLR9 antibody(ab37154)
      Immunofluorescence of TLR9 in Mouse Spleen cells using ab37154 at 10 ug/ml.

    Anti-TLR9 antibody (ab37154)参考文献

    This product has been referenced in:
    • Hua F  et al. Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone. J Neuroinflammation 8:42 (2011). WB, IHC-FoFr ; Mouse . Read more (PubMed: 21549006) »

    See 1 Publication for this product

    发表研究结果有使用 ab37154？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    提交 ab37154 的 abreview 提交 ab37154 的问题

    2 Abreviews or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-TLR9 antibody

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (lung)
    Gel Running Conditions
    Reduced Denaturing (Gel Bis-Tris 10%)
    Loading amount
    35 µg
    Specification
    lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 22°C
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    Abcam user community

    Verified customer

    提交于 May 18 2016

    Dear Sir, madam, Please can you give me more information about your antibody Anti-TLR9 antibody (ab37154) We like to use it in mouse paraffin section, and fixed in formalin.   My question is about antigen retrieval system (EDTA or ...

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    Thank you for contacting Abcam about ab37154. Please find below the suggested standard IHC-P protocol recommended for this antibody, however you may need to optimize it for your own specific needs. Standard Protocol: BUFFERS Working...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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