概述

  • 产品名称Anti-TLR9抗体[5G5]
    参阅全部 TLR9 一抗
  • 描述
    小鼠单克隆抗体[5G5] to TLR9
  • 特异性This antibody reacts very well in mouse, but more weakly in human. There are references available which suggest the antibody works well in human, however, customer feedback suggests results in human can be inconsistant.
  • 经测试应用适用于: IHC-P, Flow Cyt, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Dog
    不与反应: Human
  • 免疫原

    Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.

  • 阳性对照
    • RAW macrophages

性能

应用

Our Abpromise guarantee covers the use of ab16892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
Flow Cyt
WB
IHC-Fr
  • 应用说明Flow Cyt: 1/50.
    IHC-Fr: 1/50.Ab16892 stains RAW macrophages and TLR9 transfected HEK293 cells.
    IHC-P: Use at an assay dependent dilution.
    WB: 1/50. Predicted molecular weight: 116 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • 组织特异性Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
    • 序列相似性Belongs to the Toll-like receptor family.
      Contains 26 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • 细胞定位Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 289 antibody
      • CD289 antibody
      • TLR 9 antibody
      • TLR9 antibody
      • TLR9_HUMAN antibody
      • Toll like receptor 9 antibody
      • Toll like receptor 9 isoform A precursor antibody
      • Toll like receptor 9 isoform B antibody
      • Toll-like receptor 9 antibody
      see all

    Anti-TLR9 antibody [5G5] (ab16892)参考文献

    This product has been referenced in:
    • Machida H  et al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant 25:2530-537 (2010). Read more (PubMed: 20181802) »
    • Ilversaro et al Toll like receptor 9 agonists stimulate prostate cancer invasion in vitro The Prostate 67:774-781 (2007). Read more (PubMed: ) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your inquiry.

    The products ab16892 and ab58864 are both from the same clone [5G5] and have the same immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc.The immunogen for both is as...

    Read More

    Thank you very much for your interest in our products.

    To our knowledge,none of our TLR6 or TLR9 antibodies havebeen tested in bovine. Since we have over 90,000 products we cannot store multiple vial sizes for testing. Therefore, I can offer ...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (pancreatic)
    Specification pancreatic
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH9
    Permeabilization No
    Username

    Dr. Ilka Werner-Martini

    Verified customer

    提交于 Oct 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"