概述

  • 产品名称
  • 描述
    兔多克隆抗体to TLR8
  • 特异性
    ab24185 reacts with TLR8 protein.
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) corresponding to 16 amino acids near the middle of human TLR8 (within the Leucine-rich repeat between residues 772 and 823).

  • 阳性对照
    • Daudi cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab24185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 119 kDa.

靶标

  • 功能
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性
    Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 288 antibody
    • CD288 antibody
    • CD288 antigen antibody
    • MGC119599 antibody
    • MGC119600 antibody
    • TLR 8 antibody
    • Tlr8 antibody
    • TLR8_HUMAN antibody
    • Toll like receptor 8 antibody
    • Toll-like receptor 8 antibody
    see all

Anti-TLR8 antibody 图像

  • Lane 1 : Anti-TLR8 antibody (ab24185) at 0.5 µg/ml
    Lane 2 : Anti-TLR8 antibody (ab24185) at 1 µg/ml
    Lane 3 : Anti-TLR8 antibody (ab24185) at 2 µg/ml

    Lane 1 : Daudi Cell lysate
    Lane 2 : Daudi Cell lysate
    Lane 3 : Daudi Cell lysate


    Predicted band size : 119 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab25185 at 2µg/ml staining TLR8 in Daudi cells by ICC/IF

Anti-TLR8 antibody (ab24185)参考文献

This product has been referenced in:
  • Yu L  et al. Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells. Biomed Res Int 2016:9872138 (2016). Read more (PubMed: 28105439) »
  • Umiker BR  et al. Dosage of X-linked Toll-like receptor 8 determines gender differences in the development of systemic lupus erythematosus. Eur J Immunol 44:1503-16 (2014). Read more (PubMed: 24500834) »

See all 7 Publications for this product

Product Wall

Thank you for your reply.

I'm sending a free of charge vial of ab95047 on the order ***, which should arrive tomorrow.

Please keep me updated about the results using this replacement antibody, and let me know if there is anything el...

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Thank you for your call today and for letting us know about the trouble with ab24185 in Western blot.

As we discussed, I would recommend checking the membrane with Ponceau stain to make sure that the protein transferred, however I would be ha...

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The peptide was selected from somewhere within the Leucine-rich repeat between residues 772 and 823. Unfortunately, I can not get more specific than that but I hope this will help you, Please do not hesitate to contact us again if you need further ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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