概述

  • 产品名称Anti-TLR8抗体
    参阅全部 TLR8 一抗
  • 描述
    兔多克隆抗体to TLR8
  • 特异性ab24185 reacts with TLR8 protein.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) corresponding to 16 amino acids near the middle of human TLR8 (within the Leucine-rich repeat between residues 772 and 823).

  • 阳性对照
    • Daudi cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab24185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 119 kDa.

靶标

  • 功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 288 antibody
    • CD288 antibody
    • CD288 antigen antibody
    • MGC119599 antibody
    • MGC119600 antibody
    • TLR 8 antibody
    • Tlr8 antibody
    • TLR8_HUMAN antibody
    • Toll like receptor 8 antibody
    • Toll-like receptor 8 antibody
    see all

Anti-TLR8 antibody 图像

  • Lane 1 : Anti-TLR8 antibody (ab24185) at 0.5 µg/ml
    Lane 2 : Anti-TLR8 antibody (ab24185) at 1 µg/ml
    Lane 3 : Anti-TLR8 antibody (ab24185) at 2 µg/ml

    Lane 1 : Daudi Cell lysate
    Lane 2 : Daudi Cell lysate
    Lane 3 : Daudi Cell lysate


    Predicted band size : 119 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab25185 at 2µg/ml staining TLR8 in Daudi cells by ICC/IF

Anti-TLR8 antibody (ab24185)参考文献

This product has been referenced in:
  • Li B  et al. 1,25-dihydroxyvitamin D3 suppresses TLR8 expression and TLR8-mediated inflammatory responses in monocytes in vitro and experimental autoimmune encephalomyelitis in vivo. PLoS One 8:e58808 (2013). Read more (PubMed: 23516559) »
  • Makni-Maalej K  et al. The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1. J Immunol 189:4657-65 (2012). WB . Read more (PubMed: 23002436) »

See all 5 Publications for this product

Product Wall

Thank you for your reply.

I'm sending a free of charge vial of ab95047 on the order ***, which should arrive tomorrow.

Please keep me updated about the results using this replacement antibody, and let me know if there is anything el...

Read More

Thank you for your call today and for letting us know about the trouble with ab24185 in Western blot.

As we discussed, I would recommend checking the membrane with Ponceau stain to make sure that the protein transferred, however I would be ha...

Read More

The peptide was selected from somewhere within the Leucine-rich repeat between residues 772 and 823. Unfortunately, I can not get more specific than that but I hope this will help you, Please do not hesitate to contact us again if you need further ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"