The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Predicted molecular weight: 121 kDa.
Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
组织特异性Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
序列相似性Belongs to the Toll-like receptor family. Contains 27 LRR (leucine-rich) repeats. Contains 1 TIR domain.
细胞定位Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
Flow cytometric analysis of TLR7 antibody in Human monocytes using 2µg of ab107605 (red) and 2µg of Mouse IgG1 isotype control antibody (green). An anti-Mouse IgG PE conjugated secondary antibody.
Anti-TLR7 antibody [IMG4G6] (ab107605)参考文献
has not yet been referenced specifically in any publications.