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Our Abpromise guarantee covers the use of ab45371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).|
|Flow Cyt||Use 2-5µg for 106 cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Image courtesy of an anonymous AbReview
Primary incubation: 18 hours at 4°C
Flow cytometric analysis of human PBMCs labeling TLR7 with ab45371 at 0.5 μg/ml (pink) compared with a rabbit isotype control (green) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (shaded).
RAW 246.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for TLR7 (green) using ab45371 at a 1/10 dilution in ICC/IF. Nuclei were counterstained with DAPI (blue). Alpha-tubulin was counterstained with Dylight 550 (red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"