概述

  • 产品名称Anti-TLR7抗体
    参阅全部 TLR7 一抗
  • 描述
    兔多克隆抗体to TLR7
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    LKLEELDISKNSLSFLPSGVFDGMPPNLKNLSLAKNGLKSFSWKKLQCLK N

    conjugated to KLH, corresponding to amino acids 650-700 of Human TLR7.

  • 阳性对照
    • Ramos cells.

性能

应用

Our Abpromise guarantee covers the use of ab13732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use at a concentration of 0.5 - 2 µg/1 x 106 Ramos cells.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • 组织特异性Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
    • 序列相似性Belongs to the Toll-like receptor family.
      Contains 27 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • 细胞定位Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.
    • Information by UniProt
    • 数据库链接
    • 别名
      • PRO285 antibody
      • TLR 7 antibody
      • Tlr7 antibody
      • TLR7_HUMAN antibody
      • Toll like receptor 7 antibody
      • Toll-like receptor 7 antibody
      • UNQ248 antibody
      see all

    Anti-TLR7 antibody 图像

    • Intracellular staining by FACS analysis of TLR7 in Ramos cells (A) using ab13732 at 1µg/ml.  Histogram B shows the rabbit isotype control.

    Anti-TLR7 antibody (ab13732)参考文献

    This product has been referenced in:
    • Fahey LM  et al. Reversal of human papillomavirus-specific T cell immune suppression through TLR agonist treatment of Langerhans cells exposed to human papillomavirus type 16. J Immunol 182:2919-28 (2009). Flow Cyt ; Human . Read more (PubMed: 19234187) »

    See 1 Publication for this product

    Product Wall

    Application Flow Cytometry
    Sample Human Cell (Ramos B cell line)
    Specification Ramos B cell line
    Fixation Paraformaldehyde
    Permeabilization Yes - saponin
    Username

    Abcam user community

    Verified customer

    提交于 Jan 29 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Splenocytes)
    Specification Splenocytes
    Permeabilization No
    Username

    Dr. Karppiah Muthumani

    Verified customer

    提交于 Dec 07 2006

    Thank you for your enquiry. As you know proteins are made in the intracellular space. Thus some portion of the protein (including the extracellular epitope) may be expressed intracellularly, or trapped inside the cell - not having made it to the sur...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"