Anti-TLR5抗体[85B152.5] (Phycoerythrin) (ab45119)

概述

  • 产品名称Anti-TLR5抗体[85B152.5] (Phycoerythrin)
    参阅全部 TLR5 一抗
  • 描述
    小鼠单克隆抗体[85B152.5] to TLR5 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH corresponding to a portion of human TLR5.

  • 阳性对照
    • Ramos or ThP1 cells.

性能

应用

Our Abpromise guarantee covers the use of ab45119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 97 kDa.
Flow Cyt Use 0.5µg for 101 cells. Fixation might improve staining.



ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
  • 疾病相关Systemic lupus erythematosus 1
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 翻译后修饰Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
    Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ10052 antibody
    • MGC126430 antibody
    • MGC126431 antibody
    • SLEB1 antibody
    • TIL 3 antibody
    • TIL3 antibody
    • TLR 5 antibody
    • Tlr5 antibody
    • TLR5_HUMAN antibody
    • Toll like receptor 5 antibody
    • Toll like receptor 5 precursor antibody
    • Toll-like receptor 5 antibody
    • Toll/interleukin 1 receptor like protein 3 antibody
    • Toll/interleukin-1 receptor-like protein 3 antibody
    see all

Anti-TLR5 antibody [85B152.5] (Phycoerythrin) 图像

  • ab45119 used at 5 µg/106 cells staining ThP1 cells by intracellular FACS analysis. The shaded histogram represents ThP1 cells alone, green represents isoytpe control and pink represents TLR5 antibody.

Anti-TLR5 antibody [85B152.5] (Phycoerythrin) (ab45119)参考文献

This product has been referenced in:
  • Correale J & Farez M Helminth Antigens Modulate Immune Responses in Cells from Multiple Sclerosis Patients through TLR2-Dependent Mechanisms. J Immunol 183:5999-6012 (2009). IHC-P, Flow Cyt ; Human . Read more (PubMed: 19812189) »
  • François S  et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad. J Immunol 174:3633-42 (2005). Read more (PubMed: 15749901) »

See all 2 Publications for this product

Product Wall

Thank you for your call last week, and I sincerely apologize for the delay in getting back to you.

Ihave justreceived more information about using ab45119 in flow cytometry with a surface staining protocol, and this protocol is attached to th...

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Code de remise : 100%ABR-T7TGM Date d’expiration :  18. April 2012 Je suis ravie que vous souhaitiez participer a notre offre et tester ab45119 chez le macaque. Le code de réduction correspondant est 100%ABR-T7TGM. Ce code de réduction est utilisable s...

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I am writing to follow up on an issue with two of our products, the TLR5 antibody ab45119 and the Nanog antibody ab80892. According to our accounting department, these antibodies were purchased on orders *** and *** but were not working well for you, a...

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Antibody could be used with and without fixation. Fixation could be done to compare the intra- and extracellular TLR5 presence. I have changed the wording in the application notes. To further clarify, whereas TLR5 is a cell surface receptor, TLRs...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"