The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明FACS (intracellular): Use 2-5 µg for 106 cells.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 103 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
组织特异性Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
疾病相关Systemic lupus erythematosus 1
序列相似性Belongs to the Toll-like receptor family. Contains 22 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 TIR domain.
翻译后修饰Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13875
Anti-TLR5 antibody [85B152.5] (ab13875)参考文献
This product has been referenced in:
Hwang SH et al. Toll like receptor 3 & 4 responses of human turbinate derived mesenchymal stem cells: stimulation by double stranded RNA and lipopolysaccharide. PLoS One9:e101558 (2014).
Read more (PubMed: 25004159) »