概述

  • 产品名称Anti-TLR5抗体[85B152.5]
    参阅全部 TLR5 一抗
  • 描述
    小鼠单克隆抗体[85B152.5] to TLR5
  • 经测试应用适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH.

  • 阳性对照
    • Ramos cell lysate, mouse RAW cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab13875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
WB
  • 应用说明FACS (intracellular): Use 2-5 µg for 106 cells.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 103 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
    • 组织特异性Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
    • 疾病相关Systemic lupus erythematosus 1
    • 序列相似性Belongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.
    • 翻译后修饰Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.
      Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ10052 antibody
      • MGC126430 antibody
      • MGC126431 antibody
      • SLEB1 antibody
      • TIL 3 antibody
      • TIL3 antibody
      • TLR 5 antibody
      • Tlr5 antibody
      • TLR5_HUMAN antibody
      • Toll like receptor 5 antibody
      • Toll like receptor 5 precursor antibody
      • Toll-like receptor 5 antibody
      • Toll/interleukin 1 receptor like protein 3 antibody
      • Toll/interleukin-1 receptor-like protein 3 antibody
      see all

    Anti-TLR5 antibody [85B152.5] 图像

    • All lanes : Anti-TLR5 antibody [85B152.5] (ab13875) at 2 µg/ml

      Lane 1 : Ramos cell lysate
      Lane 2 : Raw cell lysate


      Predicted band size : 103 kDa
    • Intracellular FACS analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13875

    Anti-TLR5 antibody [85B152.5] (ab13875)参考文献

    This product has been referenced in:
    • Hwang SH  et al. Toll like receptor 3 & 4 responses of human turbinate derived mesenchymal stem cells: stimulation by double stranded RNA and lipopolysaccharide. PLoS One 9:e101558 (2014). Flow Cyt ; Human . Read more (PubMed: 25004159) »
    • Wang M  et al. Microbial hijacking of complement-toll-like receptor crosstalk. Sci Signal 3:ra11 (2010). Read more (PubMed: 20159852) »

    See all 5 Publications for this product

    Product Wall

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    Thank you for contacting us. This is a link to our recommended intracellular FC staining protocol: http://www.abcam.com/index.html?pageconfig=resource&rid=11448 I hope this information is helpful to you. Please do not hesitate to contact us if ...

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    I would like to confirm that both ab13875 and ab13876 recognise an intracellular portion of TLR5 and therefore need permeabilization. I'm afraid we do not have other antibodies tested in FACS against TLR5. However, ab1654 is raised against aa151-1...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"