Anti-TLR4/MD2 Complex抗体[MTS510] (Phycoerythrin) (ab95563)

概述

  • 产品名称Anti-TLR4/MD2 Complex抗体[MTS510] (Phycoerythrin)
    参阅全部 TLR4/MD2 Complex 一抗
  • 描述
    大鼠单克隆抗体[MTS510] to TLR4/MD2 Complex (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Toll-like receptor 4 (TLR4)/MD-2 complex of mouse origin

  • 阳性对照
    • 3-day thioglycolate-induced BALB/c macrophages; mouse peritoneal exudate cells

性能

应用

Our Abpromise guarantee covers the use of ab95563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.



ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域The TIR domain mediates interaction with NOX4.
  • 翻译后修饰N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • ESOP 1 antibody
    • ESOP1 antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • LY 96 antibody
    • LY96 antibody
    • Lymphocyte antigen 96 antibody
    • md 2 antibody
    • MD 2 protein antibody
    • MD2 protein antibody
    • Myeloid differentiation protein 2 antibody
    • Protein MD 2 antibody
    • Protein MD2 antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4/MD2 Complex antibody [MTS510] (Phycoerythrin) 图像

  • Staining of 3-day thioglycolate-induced BALB/c macrophages with 0.25 µg of Rat IgG2a ? Isotype Control PE (open histogram) or 0.25 µg of ab95563 (filled histogram). Cells were pre-incubated with Anti-Mouse CD16/CD32. Total viable cells were used for analysis.

Anti-TLR4/MD2 Complex antibody [MTS510] (Phycoerythrin) (ab95563)参考文献

This product has been referenced in:
  • Chabot S  et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). Read more (PubMed: 16547265) »
  • Mortaz E  et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006). Read more (PubMed: 16413386) »

See all 4 Publications for this product

Product Wall


From the antibodies you have been looking at, I would suggest ab45126 would be most suitable depending on the application you hope to use the antibody in. The antibody ab95563 is directed against the mouse Toll-like receptor 4 (TLR4)/MD-2. The hom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"