Anti-TLR4抗体[HTA125] (Phycoerythrin) (ab11227)

概述

• 产品名称Anti-TLR4抗体[HTA125] (Phycoerythrin)
  参阅全部 TLR4 一抗
• 描述
  小鼠单克隆抗体[HTA125] to TLR4 (Phycoerythrin)
• 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
• 特异性ab11227 recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS.
• 经测试应用适用于: Flow Cytmore details
• 种属反应性
  与反应: Human, Rhesus monkey
  
• 免疫原

  Ba/F3 cell line expressing human TLR4.

• 阳性对照
  • Human monocytes and macrophages/whole blood.
• 常规说明

  This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

  For Flow Cytometry: Use 10 µl of undiluted antibody to label 10⁶ cells or 100 µl whole blood.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C.
• 存储溶液Preservative: 0.09% Sodium Azide
  Constituents: 1% BSA, PBS
• Concentration information loading...
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号HTA125
• 骨髓瘤Sp2/0
• 同种型IgG2a
• 研究领域
  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Immunology
  • Immunoglobulins
  • Receptors

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Adaptive Immunity
  • Regulatory T Cells

  • Immunology
  • Innate Immunity
  • TLR Signaling

相关产品

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

应用

靶标

• 功能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
• 组织特异性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
• 疾病相关Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
• 序列相似性Belongs to the Toll-like receptor family.
  Contains 18 LRR (leucine-rich) repeats.
  Contains 1 LRRCT domain.
  Contains 1 TIR domain.
• 结构域The TIR domain mediates interaction with NOX4.
• 翻译后修饰N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
• 细胞定位Membrane.

• Target information above from: UniProt accession O00206 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 7099 Human
  • Omim: 603030 Human
  • SwissProt: O00206 Human
  • Unigene: 174312 Human
• 别名
  • ARMD10 antibody
  • CD284 antibody
  • CD284 antigen antibody

  • Homolog of Drosophila toll antibody
  • hToll antibody
  • TLR 4 antibody
  • TLR4 antibody
  • TLR4_HUMAN antibody
  • TOLL antibody
  • Toll like receptor 4 antibody
  • Toll-like receptor 4 antibody

  see all