Anti-TLR4抗体[HTA125] (Phycoerythrin) (ab11227)

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概述

  • 产品名称
    Anti-TLR4抗体[HTA125] (Phycoerythrin)
    参阅全部 TLR4 一抗
  • 描述
    小鼠单克隆抗体[HTA125] to TLR4 (Phycoerythrin)
  • 宿主
    Mouse
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human, Rhesus monkey
  • 免疫原

    Ba/F3 cell line expressing human TLR4.

  • 阳性对照
    • Human monocytes and macrophages/whole blood.
  • 常规说明

    This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

    TLR4 expression on U937 cells can be upregulated following stimulation with e.g. LPS or IFN gamma.

性能

应用

Our Abpromise guarantee covers the use of ab11227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/1.

Use 10 µl of undiluted antibody to label 106 cells or 100 µl whole blood.

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性
    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关
    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域
    The TIR domain mediates interaction with NOX4.
  • 翻译后修饰
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • ARMD10 antibody
      • CD284 antibody
      • CD284 antigen antibody
      • Homolog of Drosophila toll antibody
      • hToll antibody
      • TLR 4 antibody
      • TLR4 antibody
      • TLR4_HUMAN antibody
      • TOLL antibody
      • Toll like receptor 4 antibody
      • Toll-like receptor 4 antibody
      see all

    图片

    • Flow Cytometry - Anti-TLR4 antibody [HTA125] (Phycoerythrin) (ab11227)
      Flow Cytometry - Anti-TLR4 antibody [HTA125] (Phycoerythrin) (ab11227)
      Staining of U937 cells with ab11227.
    • Flow Cytometry - Anti-TLR4 antibody [HTA125] (Phycoerythrin) (ab11227)
      Flow Cytometry - Anti-TLR4 antibody [HTA125] (Phycoerythrin) (ab11227)
      Ab11227 staining on human peripheral blood monocytes.

    文献

    ab11227 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab11227？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    2 reviews or Q&A

    10
    Votes: Highest First

    Could you please tell me the concentration of AB11227, Lot 142704. In addition could you please let me know which dilution of this antibody you recommend for flow cytometry?

    Thank you for your email. We are unable to measure the concentration of our PE conjugates, so I cannot give you precise details. However, the concentration of the antibody will be approximately 0.1mg/ml. I hope this information helps, please do n...

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    Can you tell me the percentage of monocytes that express TLR4 using this antibody in FACS on live cells? Can you provide me with a figure comparing a plot using the antibody versus an isotype control?

    Thank you for your enquiry. Expression of TLR4 on normal resting monocytes is low and for this reason ab11227 is routinely QC'd on the U937 cell line which demonstrates a reasonable level of expression. I can attach an image here so I will send ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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