Anti-TLR4抗体[HTA125] (FITC) (ab8378)

概述

  • 产品名称Anti-TLR4抗体[HTA125] (FITC)
    参阅全部 TLR4 一抗
  • 描述
    小鼠单克隆抗体[HTA125] to TLR4 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Guinea pig, Human
  • 免疫原

    Ba/F3 cell line expressing TLR4

性能

应用

Our Abpromise guarantee covers the use of ab8378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. Use 10 µl of this concentration to label 106 cells or 100µl whole blood.



ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域The TIR domain mediates interaction with NOX4.
  • 翻译后修饰N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody [HTA125] (FITC) 图像

  • ab8378 staining TLR4 (FITC) in human peripheral blood monocytes by Flow Cytometry analysis.

Anti-TLR4 antibody [HTA125] (FITC) (ab8378)参考文献

This product has been referenced in:
  • Velot E  et al. Activation of the adenosine-A3 receptor stimulates matrix metalloproteinase-9 secretion by macrophages. Cardiovasc Res : (2008). Read more (PubMed: 18653544) »
  • Falcone S  et al. Activation of acid sphingomyelinase and its inhibition by the nitric oxide/cyclic guanosine 3',5'-monophosphate pathway: key events in Escherichia coli-elicited apoptosis of dendritic cells. J Immunol 173:4452-63 (2004). Read more (PubMed: 15383576) »

See all 2 Publications for this product

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We do not know whether this antibody will cross react with mouse tissue. We do not have any trial samples for testing purposes, but would be interested to know whether this antibody cross reacts with mouse. If you decide to purchase this antibody, plea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"