Anti-TLR4抗体[HTA125] (Biotin) (ab17232)

概述

  • 产品名称Anti-TLR4抗体[HTA125] (Biotin)
    参阅全部 TLR4 一抗
  • 描述
    小鼠单克隆抗体[HTA125] to TLR4 (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Human, Cynomolgus Monkey, Rhesus monkey
  • 免疫原

    TLR4 expressing Ba/F3 cells.

  • 阳性对照
    • Human peripheral blood monocytes
  • 常规说明


    Ab17232 reacts preferentially, especially in flow cytometry, with human TLR4 associated with MD-2. It is a LR4 function blocking antibody that is useful for studies on the role of TR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.

性能

应用

Our Abpromise guarantee covers the use of ab17232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50.

靶标

  • 功能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域The TIR domain mediates interaction with NOX4.
  • 翻译后修饰N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody [HTA125] (Biotin) (ab17232)参考文献

ab17232 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Unfortunately I have not been able to obtain a specific protocol for this antiserum. However, we do have an excellent IHC-Fr protocol located at the following link: http://www.abcam.com/index.html?pageconfig=view_protocol...

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Thank you for your enquiry. Yes, ab17232 has been tested for application in IHC-frozen sections and tested for cross-reactivity with Human, Cynomolgus Monkey, Marmoset Monkey and Rhesus monkey. I would suggest a dilution range of 1:20-1:200, and optimi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"