• 产品名称Anti-TLR4抗体
    参阅全部 TLR4 一抗
  • 描述
    小鼠单克隆抗体to TLR4
  • 特异性TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.
  • 经测试应用适用于: ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: PMNFIQPGAF KEIRLHKLTL RNNFDSLNVM KTCIQGLAGL EVHRLVLGEF RNEGNLEKFD KSALEGLCNL TIEEFRLA, corresponding to amino acids 214-292 of Human TLR4 with GST tag (NP_612564)

  • 阳性对照
    • Human spleen tissue lysate.



Our Abpromise guarantee covers the use of ab89456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.


  • 功能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域The TIR domain mediates interaction with NOX4.
  • 翻译后修饰N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody (ab89456)参考文献

ab89456 has not yet been referenced specifically in any publications.

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Thank you very much for your call today, and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab89455 on the order ***, which should arrive tomorrow.

Please keep me upda...

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Thank you for contacting Abcam.

I am sorry about the issue you have been having with ab89456 in ELISA. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on rabbit samples . If we cannot resolv...

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