概述

  • 产品名称
  • 描述
    兔多克隆抗体to TLR4
  • 特异性
    TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate. We have obtained both positive and negative feedback from researchers using this antibody with rat samples (see Abreviews). Due to the inconsistency, we have removed rat as a guaranteed species and welcome any further feedback from researchers using this antibody.
  • 经测试应用
    适用于: IHC-P, WB, ELISA, ICC/IF, ICC, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human TLR4 aa 420-435.
    Sequence:

    GLEQLEHLDFQ HSNLK


    Database link: O00206

  • 阳性对照
    • overexpressed recombinant protein

性能

应用

Our Abpromise guarantee covers the use of ab13556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50.
WB 1/500.

Use primary at 1/500 dlution in 1xTBS. Incubate at 4C for 18 hours. Secondary should be incubated at 1 hour at room temperature.

ELISA Use at an assay dependent concentration. PubMed: 24952384
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性
    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关
    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域
    The TIR domain mediates interaction with NOX4.
  • 翻译后修饰
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody 图像

  • Anti-TLR4 antibody (ab13556) at 1/500 dilution + TLR4 transfected Baculovirus-Insect whole cell lysate at 10 µg

    Secondary
    Anti-Rabbit HRP conjugate at 1/2000 dilution

    Exposure time : 6 minutes
  • ab13556 staining TLR4 in human mouse spleen tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab13556 positively staining formaldehyde fixed rat primary microglia cells (1/50), used in conjunction with goat anti rabbit Alex Fluor® 488 (1/250). 
    This image is courtesy of an Abreview submitted on 3 October 2005. We do not have any further information relating to this image.

  • ab13556 diluted 1/100 detecting TLR4 transfected mouse CHO cells by flow cytometry.   The cells were prepared by treatment with collagenase and incubated with the primary antibody for 1 hour at 22°C.  An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody.  Cells gated on live.

    See Abreview

  • ab13556 at 1/20000 dilution staining TLR4 in mouse colitis colon tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated goat polyclonal to rabbit IgG was used as secondary at 1/10 dilution.

  • Flow cytometry analysis of Human monocytic Leukemia cells (THP-1). Cells were fixed with 2% Formaldehyde for 10 minutes at room temperature. Ab13556 was used at  2 µg/106 cells for 60 minutes at 37°C (green). Goat Anti- Rabbit Dylight 488 was used as a secondary antibody at 1:200 dilution for 40minutes at 37°C. Isotype control was Rabbit IgG under the same conditions (blue).

Anti-TLR4 antibody (ab13556)参考文献

This product has been referenced in:
  • Zhang H  et al. Toll-Like Receptor 4-Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Inflammatory Development of Polymyositis by Mediating Interferon-? and Interleukin-17A in Humans and Experimental Autoimmune Myositis Mouse Model. Front Neurol 8:132 (2017). IHC-P . Read more (PubMed: 28446897) »
  • Shen W  et al. High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4. Mol Med Rep 15:2530-2536 (2017). IHC-Fr ; Human . Read more (PubMed: 28260034) »

See all 27 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cynomolgus Monkey Tissue lysate - other (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
300 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 02 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Mouse Tissue sections (frozen sections from 13.5dpc mouse embryo and plac)
Specification
frozen sections from 13.5dpc mouse embryo and plac
Permeabilization
No
Fixative
Acetone
Username

Mrs. Ira Rostovsky

Verified customer

提交于 Feb 16 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Marmoset (common) Tissue sections (Caudate)
Specification
Caudate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 21 2014

Thank you for contacting us.

I have received a response from the lab:

The antibody amplifier(TM) refers to a platform that was used. I have requestedthis productto be added to our catalog. I will let you know if this is possible. <...

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We do not sell a blocking peptide for this product unfortunately.

Sorry I couldn't be more helpful here. Please contact us again if you have any further questions.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting Abcam.

I am very sorry to hear about the troubles which you have been experiencing using this product and wish to thank you for providing the details of your protocol.

Our products are covered by Abpromise gua...

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Thank you very much for your interest in our TLR4 antibodies.
Based on the homology between the Sheep sequence (UniProt: B5DC89) and their immunogens, I would recommend ab47093 or ab13556, both of which are 94% homologous. Our antibody ab22048 als...

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Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any TLR4 antibody tested in the s...

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1-10 of 42 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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