Anti-TLR4抗体[76B357.1] (Phycoerythrin) (ab45104)

概述

  • 产品名称Anti-TLR4抗体[76B357.1] (Phycoerythrin)
    参阅全部 TLR4 一抗
  • 描述
    小鼠单克隆抗体[76B357.1] to TLR4 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Horse, Cow, Cat, Pig, Chimpanzee, Baboon, Chinese Hamster
  • 免疫原

    This antibody was developed against a portion of amino acids 100-200 of human TLR4.

  • 阳性对照
    • Ramos or ThP1 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab45104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 2µl for 106 cells.

ab91532-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • 组织特异性Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • 疾病相关Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 结构域The TIR domain mediates interaction with NOX4.
  • 翻译后修饰N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARMD10 antibody
    • CD284 antibody
    • CD284 antigen antibody
    • Homolog of Drosophila toll antibody
    • hToll antibody
    • TLR 4 antibody
    • TLR4 antibody
    • TLR4_HUMAN antibody
    • TOLL antibody
    • Toll like receptor 4 antibody
    • Toll-like receptor 4 antibody
    see all

Anti-TLR4 antibody [76B357.1] (Phycoerythrin) 图像

  • ab45104 staining Ramos cells, at 5 µg/106 cells, by flow analysis. Shaded histogram represents cells without anti-TLR4 antibody, green represents isotype control and red represents anti-TLR4 antibody.

Anti-TLR4 antibody [76B357.1] (Phycoerythrin) (ab45104)参考文献

This product has been referenced in:
  • Brandau S  et al. Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes. PLoS One 9:e106903 (2014). Flow Cyt ; Human . Read more (PubMed: 25238158) »
  • Hill RP  et al. Human hair follicle dermal cells and skin fibroblasts show differential activation of NF-?B in response to pro-inflammatory challenge. Exp Dermatol 21:158-60 (2012). Flow Cyt ; Human . Read more (PubMed: 22141576) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Treatment TLR4 knockout
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2014

Yes, sometimes PE conjugated antibodies can give non-specific binding and result in a higher level of positive cells than other conjugates, You may need to titrate the antibody down to reduce background and establish the optimal concentration for your ...

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Thank you very much for your call yesterday and for your patience while I've been in touch with the lab.

The ratio of PE to IgG is approximately 1:1.

I hope that this information will be useful, but if you have further questions or ...

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I hope you enjoyed the German Society of Virology meeting in Essen. My colleague attending this meeting forwarded your inquiry to me.

I am happy to confirm that this antibody was tested with mouse splenocytes. I attached the image for your in...

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The FACS data suggests the antibody recognizes an epitope that is not obscured when TLR4 is in complex with MD2. This is assuming that the TLR4/MD2 complex is how TLR4 typically exists on those samples. There are several references on the datasheet of ...

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Thank you for your interest in ab45104.

We do not have any small samples, just the one size.

The antibody was validated for mouse reactivity using the unconjugated version of this clone. I have attached the image of the FACS data....

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I have issued a free of charge replacement for one vial of ab91532. To check the status of the order please contact our Customer Service team and reference this number. Please be aware this product is currently out of stock. Expect delivery around Dece...

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Thank you for contacting us. The product ab7002 is not an isotype control, but a secondary antibody that binds to antibodies raised in mouse (such as ab45104). I am not certain which ED9-antibody (ab34086 Anti-SIRP alpha antibody [ED9] ??) you ar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"