Anti-TLR3抗体[TLR3.7] (Biotin) (ab17264)

概述

  • 产品名称Anti-TLR3抗体[TLR3.7] (Biotin)
    参阅全部 TLR3 一抗
  • 描述
    小鼠单克隆抗体[TLR3.7] to TLR3 (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Flow Cyt, WB, IHC-Fr, Functional Studies, ICC/IF, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Dog, Human
  • 免疫原

    Human Flag-tagged TLR3 stably expressed by Ba/F3 cells.

  • 阳性对照
    • Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells.

性能

应用

Our Abpromise guarantee covers the use of ab17264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/50. Predicted molecular weight: 100 kDa.
IHC-Fr 1/50.
Functional Studies Use at an assay dependent concentration.  Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production
ICC/IF 1/50.
IP 1/50.
IHC-P 1/50.

靶标

  • 功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • 疾病相关Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • 结构域ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • 翻译后修饰Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • 细胞定位Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Anti-TLR3 antibody [TLR3.7] (Biotin) (ab17264)参考文献

This product has been referenced in:
  • Matsumoto M  et al. Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 171:3154-62 (2003). ICC/IF, Flow Cyt, IP ; Human . Read more (PubMed: 12960343) »
  • Matsumoto M  et al. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 293:1364-9 (2002). Read more (PubMed: 12054664) »

See all 3 Publications for this product

Product Wall

I heard back from our lab that the most recent lot of ab12085 had an endotoxin level of 0.02EU/ml. The ab17264 has not been tested for endotoxin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"