概述

  • 产品名称
  • 描述
    兔多克隆抗体to TLR3
  • 经测试应用
    适用于: IHC-P, ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A peptide corresponding to 15 amino acids near the carboxy terminus of human TLR3 (Peptide available as ab125950).

  • 阳性对照
    • WB: Daudi cell lysate. IHC-P: Human and mouse spleen. ICC/IF: EL4 cells.

性能

应用

Our Abpromise guarantee covers the use of ab62566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).Can be blocked with TLR3 peptide (ab125950).

靶标

  • 功能
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性
    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • 疾病相关
    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • 结构域
    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • 翻译后修饰
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • 细胞定位
    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all

Anti-TLR3 antibody 图像

  • Paraffin-embedded mouse spleen tissue stained for TLR3 using ab62566 at 2 µg/ml in immunohistochemical analysis.

  • Lane 1 : Anti-TLR3 antibody (ab62566) at 1 µg/ml
    Lane 2 : Anti-TLR3 antibody (ab62566) at 2 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 104 kDa
    Observed band size : 104 kDa
  • Paraffin-embedded human spleen tissue stained for TLR3 using ab62566 at 5 µg/ml in immunohistochemical analysis.

  • Immunofluorescence of TLR3 in El4 cells using ab62566 at 10 ug/ml.

  • ab62566, at 1µg/ml, staining TLR3 in EL4 cells by immunocytochemistry.

Anti-TLR3 antibody (ab62566)参考文献

This product has been referenced in:
  • Shan M  et al. TIR-Domain-Containing Adapter-Inducing Interferon-ß (TRIF) Is Essential for MPTP-Induced Dopaminergic Neuroprotection via Microglial Cell M1/M2 Modulation. Front Cell Neurosci 11:35 (2017). WB . Read more (PubMed: 28275337) »
  • Ding X  et al. TLR4 signaling induces TLR3 up-regulation in alveolar macrophages during acute lung injury. Sci Rep 7:34278 (2017). Read more (PubMed: 28198368) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Cynomolgus Monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
300 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 05 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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提交于 Nov 02 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Nov 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (whole embryo sections)
Specification
whole embryo sections
Permeabilization
Yes - 0.1% triton x100 in TBS
Fixative
Formaldehyde
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提交于 Aug 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 4%
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Formaldehyde
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提交于 Jun 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary lung epithelial cell)
Loading amount
20 µg
Specification
Primary lung epithelial cell
Treatment
10 ng/ml Poly I poly C
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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提交于 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (7,5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Oct 16 2012

Muchas gracias pos facilitarnos estos detalles. Estudiando el protocolo, todo parece correcto, no tenemos ninguna sugerencia que hacer. Es posible que el lote de anticuerpo concreto que recibisteis no funcionara correctamente.

Lamento mucho l...

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Gracias por contactarnos. Lamento mucho que el cliente esté teniendo problemas con nuestros productos, y agradezco enormemente que nos haya mandado las imágenes y la información del protocolo.

Sin embargo, para tratar de ...

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Thank you for contacting us.
We do not publish this information for all antibodies. The antibodies should detect denatured and reduced form of protein, if information is not given on the datasheet. If these does detect native form then it will be ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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