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Our Abpromise guarantee covers the use of ab62566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).Can be blocked with TLR3 peptide (ab125950).|
Immunohistochemistry of TLR3 in human spleen tissue with TLR3 antibody at 5 µg/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"