概述

  • 产品名称
    Anti-TLR2抗体[T2.5]
    参阅全部 TLR2 一抗
  • 描述
    小鼠单克隆抗体[T2.5] to TLR2
  • 宿主
    Mouse
  • 特异性
    The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. Negative control: CHO cells.
  • 经测试应用
    适用于: Functional Studies, ICC/IF, WB, IP, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse TLR2.
    Database link: Q9QUN7

  • 阳性对照
    • Flow Cyt: RAW 264.7 cells. WB: Human lymph node tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab16894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Mice were injected i.p. with 1 mg ab16894, after 1 hour incubation mice were challenged; 5 µg/ml was added to cell culture.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 25404049
IP Use at an assay dependent concentration.

40 µg cleared protein was incubated with 2 µg ab16894 for 1 hour at 4 °C.

Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

4 x 104 leukocytes/ml were stained for 30 minutes at 4 °C.

 

IHC-Fr Use at an assay dependent concentration.

6 µm sections were fixed with acetone. Sections were blocked with goat serum and exposed overnight.

靶标

  • 功能
    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • 组织特异性
    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 翻译后修饰
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

图片

  • Detection of TLR2 in RAW cells. Red, black and blue line represent the isotype control, cells only and ab16894 at 10 μg/ml, respectively.

  • Effect of anti-TLR antibodies on TNF production in a whole blood model.

  • Anti-TLR2 antibody [T2.5] (ab16894) at 1/100 dilution + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 89 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

文献

This product has been referenced in:
  • Nguyen CT  et al. Inhibitory effects of superoxide dismutase 3 on Propionibacterium acnes-induced skin inflammation. Sci Rep 8:4024 (2018). Read more (PubMed: 29507345) »
  • Jiang C  et al. The Expression of Toll-like receptors in eutopic and ectopic endometrium and its implication in the inflammatory pathogenesis of adenomyosis. Sci Rep 7:7365 (2017). Read more (PubMed: 28779087) »

See all 5 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (human monocytic cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
human monocytic cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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提交于 Aug 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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