重组
RabMAb

Anti-TLR2抗体[EPNCIR133] (ab108998)

概述

  • 产品名称
    Anti-TLR2抗体[EPNCIR133]
    参阅全部 TLR2 一抗
  • 描述
    兔单克隆抗体[EPNCIR133] to TLR2
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TLR2 aa 1-600 (extracellular).
    (Peptide available as ab177683)

  • 阳性对照
    • Recommend using a positive control of LPS stimulated THP1 cells, as the signal can be very weak in some tissues or cells. THP1, A549, Ramos, and SW480 cell lysates.
  • 常规说明

     

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Segal.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).

Recommend using the positive control of LPS stimulated THP1 cells, as the signal can be very weak in some tissues or cells. Recommend diluting antibody in 1% BSA for better results.

  • 应用说明
    Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • 功能
      Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
    • 组织特异性
      Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
    • 序列相似性
      Belongs to the Toll-like receptor family.
      Contains 14 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • 翻译后修饰
      Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    图片

    • All lanes : Anti-TLR2 antibody [EPNCIR133] (ab108998) at 1 µg/ml

      Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) +LPS Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 90 kDa
      Observed band size : 90 kDa


      Exposure time : 4 minutes

      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab108998 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • All lanes : Anti-TLR2 antibody [EPNCIR133] (ab108998) at 1/1000 dilution (purified)

      Lane 1 : THP-1 (Human acute monocytic leukemia) cell lysates
      Lane 2 : Human liver tissue lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 90 kDa
      Observed band size : 90 kDa

      Blocking and diluting buffer: 5% NFDM/TBST

      Exposure time:  Lane 1: 3 seconds
                                Lane 2: 3 minutes

    实验方案

    文献

    This product has been referenced in:
    • Karlsen M  et al. Expression of Toll-Like Receptors in Peripheral Blood Mononuclear Cells of Patients with Primary Sjögren's Syndrome. Scand J Immunol 85:220-226 (2017). Read more (PubMed: 27943374) »
    • Fei L  et al. Glycyrrhizin Ameliorate Ischemia Reperfusion Lung Injury through Downregulate TLR2 Signaling Cascade in Alveolar Macrophages. Front Pharmacol 8:389 (2017). WB ; Mouse . Read more (PubMed: 28670282) »

    See all 14 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cell (endothelial cells)
    Permeabilization
    No
    Specification
    endothelial cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 15°C
    Fixative
    Methanol
    Username

    Dr. 禎彦 沢 

    Verified customer

    提交于 Feb 29 2016

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - other (Frontal Cortex)
    Specification
    Frontal Cortex
    Blocking step
    Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 21 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (peripheral blood cells)
    Loading amount
    30 µg
    Specification
    peripheral blood cells
    Gel Running Conditions
    Reduced Denaturing (MES buffer, 4-12% Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 17 2012

    Thank you for the additional information.

    The lab let me know that they tested the antibody with C6 cells and saw one band.

    The WB protocol used by the labis as follows:

    Western Blotting

    1. Block nitrocellulose...

    Read More

    Thank you for your reply.
    I am sorry to hear that the antibody is not working with your rat samples.

    I will get in touch with the lab to see how they tested both TLR2 antibodies on rat, and let you know what I find out.

    Have yo...

    Read More

    Thank you for contacting us.

    The antibody ab108998 was raised against a recombinant protein corresponding to Human TLR2 extracellular domain.

    The sequence of the extracellular domain of human TLR2is as follows (residues 1-588): Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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