概述

  • 产品名称Anti-TLR2抗体[6C2]
    参阅全部 TLR2 一抗
  • 描述
    大鼠单克隆抗体[6C2] to TLR2
  • 经测试应用适用于: IP, ICC/IF, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse
  • 免疫原

    CHO cells expressing mouse TLR2.

性能

应用

Our Abpromise guarantee covers the use of ab11864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
ICC/IF
Flow Cyt
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    Before use in biological assays the product should be filter sterilised. Depending on the working concentration, it may need to be dialysed against tissue culture medium to remove the sodium azide added.

    Is unsuitable for WB.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
    • 组织特异性Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
    • 序列相似性Belongs to the Toll-like receptor family.
      Contains 14 LRR (leucine-rich) repeats.
      Contains 1 TIR domain.
    • 翻译后修饰Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    Anti-TLR2 antibody [6C2] 图像

    • ab11864 staining TLR2 in Mouse primary macrophage by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Acetone for 5 minutes at -20°C and blocked with 1% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/150 in PBS + 1% BSA) for 2 hours at 25°C. A Cy5®-conjugated Goat anti-rat IgG monoclonal (1/500) was used as the secondary antibody.

      See Abreview

    Anti-TLR2 antibody [6C2] (ab11864)参考文献

    This product has been referenced in:
    • Nilsen N  et al. Lipopolysaccharide and double-stranded RNA up-regulate toll-like receptor 2 independently of myeloid differentiation factor 88. J Biol Chem 279:39727-35 (2004). Read more (PubMed: 15190057) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Primary macrophage)
    Specification Primary macrophage
    Fixative Paraformaldehyde
    Permeabilization Yes - Acetone, 5 min, -20
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 26 2013

    Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. To our knowledge, ab11864 has only been tested for application in Immunoprecipitation, Immunofluorescence, and Flow Cytometry / FAC...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"