概述

  • 产品名称Anti-TLR1抗体
    参阅全部 TLR1 一抗
  • 描述
    兔多克隆抗体to TLR1
  • 特异性ab37068 recognises Toll-like receptor 1 (TLR1).
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide of 16 amino acids near the amino terminus (Human)

  • 阳性对照
    • Mouse spleen lysate IF/ICC: RAW246.7 cell line

性能

应用

Our Abpromise guarantee covers the use of ab37068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 90 kDa.
ICC/IF Use a concentration of 1 - 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
  • 序列相似性Belongs to the Toll-like receptor family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • 细胞定位Cell membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD281 antibody
    • CD281 antigen antibody
    • KIAA0012 antibody
    • LPRS5 antibody
    • rsc786 antibody
    • TIL antibody
    • TIL. LPRS5 antibody
    • Tlr1 antibody
    • TLR1_HUMAN antibody
    • Toll (Drosophila) homolog antibody
    • Toll like receptor 1 antibody
    • Toll-like receptor 1 antibody
    • Toll/interleukin-1 receptor-like protein antibody
    see all

Anti-TLR1 antibody 图像

  • Lane 1 : Anti-TLR1 antibody (ab37068) at 1 µg/ml
    Lane 2 : Anti-TLR1 antibody (ab37068) at 2 µg/ml
    Lane 3 : Anti-TLR1 antibody (ab37068) at 4 µg/ml

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 90 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab37068 at 10µg/ml staining TLR1 in mouse spleen tissue by IHC

  • ICC/IF image of ab37068 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of TLR1 in Mouse Spleen cells using ab37068 at 20 ug/ml.

Anti-TLR1 antibody (ab37068)参考文献

This product has been referenced in:
  • Hinojosa E  et al. Age-associated inflammation and toll-like receptor dysfunction prime the lungs for pneumococcal pneumonia. J Infect Dis 200:546-54 (2009). WB ; Mouse . Read more (PubMed: 19586419) »

See 1 Publication for this product

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Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.


Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

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Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

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Thank you for contacting us. Ab37068 Anti-TLR1 should not cross-react with TLR2 as there is little homology between the immunogen and TLR2. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"