The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 88 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
组织特异性Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.