概述

  • 产品名称Anti-TLK2抗体
    参阅全部 TLK2 一抗
  • 描述
    小鼠单克隆抗体to TLK2
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-126 of Human TLK2

性能

应用

Our Abpromise guarantee covers the use of ab56816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
  • 组织特异性Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC44450 antibody
    • PKU alpha antibody
    • PKU-alpha antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase tousled like 2 antibody
    • Serine/threonine-protein kinase tousled-like 2 antibody
    • TLK 2 antibody
    • tlk2 antibody
    • TLK2_HUMAN antibody
    • Tousled like kinase 2 antibody
    • Tousled-like kinase 2 antibody
    see all

Anti-TLK2 antibody 图像

  • TLK2 antibody (ab56816) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.


  • Predicted band size : 88 kDa
    TLK2 antibody (ab56816) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab56816 staining TLK2 in U2OS human osteosarcoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 0.5% Triton/PBS for 5 minutes, blocked with 10% serum for 1 hour at room temperature and then incubated with ab56816 at a 1/100 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab56816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-TLK2 antibody (ab56816)参考文献

ab56816 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS human osteosarcoma cell line)
Specification U2OS human osteosarcoma cell line
Fixative Formaldehyde
Permeabilization Yes - triton 0,5% in PBS for 5 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"