概述

  • 产品名称Anti-TLE3抗体
    参阅全部 TLE3 一抗
  • 描述
    兔多克隆抗体to TLE3
  • 经测试应用适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human TLE3.

    (Peptide available as ab109092.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Breast (Human) Tissue Lysate; MCF7 Whole Cell Lysate; MDA-MB-231 Whole Cell Lysate; Lung (Human) Tissue Lysate; A549 Whole Cell Lysate; A431 Whole Cell Lysate; HeLa Nuclear Lysate

性能

应用

Our Abpromise guarantee covers the use of ab94972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1 mg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
  • 组织特异性Placenta and lung.
  • 序列相似性Belongs to the WD repeat Groucho/TLE family.
    Contains 7 WD repeats.
  • 翻译后修饰Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E(sp1) homolog Drosophila antibody
    • Enhancer of split groucho 3 antibody
    • Enhancer of split groucho-like protein 3 antibody
    • ESG 3 antibody
    • ESG antibody
    • ESG3 antibody
    • FLJ39460 antibody
    • GRG 3 antibody
    • GRG3 antibody
    • HsT18976 antibody
    • KIAA1547 antibody
    • TLE 3 antibody
    • Tle3 antibody
    • TLE3_HUMAN antibody
    • Transducin like enhancer of split 3 antibody
    • Transducin like enhancer of split 3 homolog of Drosophila E(sp1) antibody
    • Transducin like enhancer protein 3 antibody
    • transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) antibody
    • Transducin-like enhancer protein 3 antibody
    see all

Anti-TLE3 antibody 图像

  • TLE3 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to TLE3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab94972.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 83kDa; TLE3

  • All lanes : Anti-TLE3 antibody (ab94972) at 1 µg/ml

    Lane 1 : Human breast tissue lysate - total protein (ab30090)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
    Additional bands at : 125 kDa,43 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab94972 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94972, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-TLE3 antibody (ab94972)参考文献

ab94972 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab94972.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"