TLE2 overexpression 293T裂解液(全细胞) (ab94118)

概述

  • 产品名称TLE2 overexpression 293T裂解液(全细胞)
  • 描述
    TLE2 overexpression裂解液
  • 常规说明ab94118 is a 293T cell transfected lysate in which Human TLE2 has been transiently over-expressed using a pCMV-TLE2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Tissue specificity: In all tissues examined, mostly in heart, brain, and muscle. Similarity: Belongs to the WD repeat Groucho/TLE family. Contains 6 WD repeats.

应用

Our Abpromise guarantee covers the use of ab94118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

TLE2 overexpression 293T lysate (whole cell) 图像

  • ab94118 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-TLE2 antibody (ab72634) at 1/500 dilution

    Lane 1 : TLE2 overexpression 293T lysate (whole cell) (ab94118)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

TLE2 overexpression 293T lysate (whole cell) (ab94118)参考文献

ab94118 has not yet been referenced specifically in any publications.

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