TLE2 overexpression 293T裂解物(whole cell) (ab94118)
概述
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产品名称
TLE2 overexpression 293T裂解物(whole cell) -
常规说明
ab94118 is a 293T cell transfected lysate in which Human TLE2 has been transiently over-expressed using a pCMV-TLE2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 10% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 2% Sodium lauryl sulfate, 2.3% Beta mercaptoethanol, 0.01% Bromophenol blue -
Concentration information loading...
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研究领域
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背景
Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Tissue specificity: In all tissues examined, mostly in heart, brain, and muscle. Similarity: Belongs to the WD repeat Groucho/TLE family. Contains 6 WD repeats.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94118于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94118 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-TLE2 antibody (ab72634) at 1/500 dilution
Lane 1 :TLE2 overexpression 293T lysate (whole cell) (ab94118)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94118 尚未被引用在任何文献中。