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Tissue type Plasminogen Activator (tPA) is a 68 kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin which digests fibrin and induces the dissolution of fibrin clots. Tissue type Plasminogen Activator is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo. High level of Tissue type Plasminogen Activator is a good prognostic marker for breast cancer. On the other hand, gastrointestinal cancer is accompanied by a decrease in Tissue type Plasminogen Activator.
ab108905 Tissue type Plasminogen Activator Human Chromogenic Activity Assay kit is developed to determine Human Tissue type Plasminogen Activator activity in cell culture supernatants and plasma. The assay measures the ability of Tissue type Plasminogen Activator to activate the plasminogen to plasmin in coupled or indirect assays that contain Tissue type Plasminogen Activator, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the Tissue type Plasminogen Activator enzymatic activity.
|Diluent||1 x 30ml|
|Human Plasminogen||1 unit|
|Microplate 1 96 well polystyrene microplate (12 strips of 8 wells)||1 unit|
|Plasmin Substrate||2 units|
|Sealing Tapes||3 units|
|Tissue type Plasminogen Activator Standard||1 x 32 units|
Our Abpromise guarantee covers the use of ab108905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
tPA measured in biological fluids showing activity (IU) per mL of tested sample
Time course of tPA measured in biological fluids showing optical densities
Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
Change of absorbance per min. of various concentrations of tPA standard
O.D's of various dilutions of human plasma after 20hrs using ab108905
ab108905 has not yet been referenced specifically in any publications.