ab62251 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/2000. PubMed: 21310953
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
ab62251 at 1/50 - 1/100 dilution staining Tissue Factor in Human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Tissue Factor (phospho S290) antibody (ab62251)
Anti-Tissue Factor (phospho S290) antibody (ab62251) at 1/2000 dilution + whole cell lysate prepared from transfected human endothelial cells expressing the human full length Tissue Factor (phospho S290) (treated with PAR2-agonist peptide for 90 minutes) at 20 µg/ml
Secondary Goat anti-rabbit IgG-HRP at 1/1000 dilution
Collier ME & Ettelaie C Regulation of the incorporation of tissue factor into microparticles by serine phosphorylation of the cytoplasmic domain of tissue factor. J Biol Chem286:11977-84 (2011).
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