组织Factor人Chromogenic Activity Assay试剂盒(ab108906)

概述

  • 产品名称组织Factor人Chromogenic Activity Assay试剂盒
    参阅全部 Tissue Factor 试剂盒
  • 测试
    1 x 96 well plate
  • 样品类型
    Urine, Serum, Plasma, Cell culture extracts, Tissue
  • 检测类型Quantitative
  • 种属反应性
    与反应: Human
  • 产品概述

    The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates. Inducible expression of Tissue factor in a variety of pathological conditions, including gram-negative sepsis and acute coronary syndromes, is associated with life-threatening thrombosis. In sepsis, Tissue factor expression within the vasculature leads to disseminated intravascular coagulation. Tissue factor also plays important roles in vasculogenesis, metastasis, and tumor-associated angiogenesis.

     

    ab108906 Tissue Factor Human Chromogenic Activity Assay kit is developed to determine Human Tissue Factor activity in plasma, tissue and cell culture supernatants. The assay measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 功能Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 序列相似性Belongs to the tissue factor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD142
    • CD142 antigen
    • Coagulation factor III
    • Coagulation factor III (thromboplastin tissue factor)
    • F3
    • FLJ17960
    • TF
    • TF_HUMAN
    • TFA
    • Thromboplastin
    • Tissue factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Tissue Factor Human Chromogenic Activity Assay Kit 图像

  • Colorimetric standard curve: mean of duplicates (+/- SD)

  • Standard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame: 20 min.)

  • Time course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)

  • Tissue Factor measured in various biologicals showing average concentrations

实验方案

Tissue Factor Human Chromogenic Activity Assay Kit (ab108906)参考文献

ab108906 has not yet been referenced specifically in any publications.

Product Wall


The standard should be reconstituted in 3ml water.



After arrival please store the individual components as instructed in the protocol booklet:

ab108906 may be stored until the expiration date at 2 to 8°C from the date of shipment. Store Standard, Factor VII protein, Factor X pro...

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Since tissue factor is both secreted and membrane bound, we recommend using cell lysates so the protein is properly released from the membrane.

As mentioned in the protocol under step 6.2) Cells lysates can be prepared as follows:

1. ...

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The labs says that the kit contains a low binding plate which will be better. However, otherwise the brand of the 96-well plate does not matter.

Thank you for contacting us on Friday in regards to the Tissue Factor Human Chromogenic Activity Assay Kit (ab108906).

I can now inform you that the vial of Tissue Factor Standard you received required the addition of 1.2 ml of reagent grade...

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The amount of standard is only provided on the vial label. The datasheet and the protocol bookletare only generic.
Please ask your customer tocheck the vial label, from which they can work out the volume of buffer they need to to generate a s...

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Thank you very much for contacting us.
I just wanted to let you know that I am still working on your inquiry. I am looking forthe information you requested.
I will forward to you the information as soon as I receive it. Thank you for your pat...

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I have just been informed that the tissue factor standard should be reconstituted with 1.2 ml of reagent grade water to generate a solution of 250 pM.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"