Anti-Tissue Factor抗体[TF9-10H10] (ab35807)

概述

  • 产品名称Anti-Tissue Factor抗体[TF9-10H10]
    参阅全部 Tissue Factor 一抗
  • 描述
    小鼠单克隆抗体[TF9-10H10] to组织Factor
  • 特异性This antibody reacts with reduced and native non reduced Tissue Factor. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: ELISA, ICC/IF, WB, Flow Cyt, Dot blotmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Purified native apoprotein of human Tissue Factor (47 kDa).

  • 表位This antibody recognises an epitope within the extracellular domain, locus 1.

性能

应用

Our Abpromise guarantee covers the use of ab35807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
ICC/IF
WB
Flow Cyt
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    ELISA: 1/500 - 1/5000.
    In ELISA this antibody is reactive agaisnt recombinant human tissue factor.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 33 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
    • 序列相似性Belongs to the tissue factor family.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD142 antibody
      • CD142 antigen antibody
      • Coagulation factor III (thromboplastin tissue factor) antibody
      • Coagulation factor III antibody
      • F3 antibody
      • FLJ17960 antibody
      • TF antibody
      • TF_HUMAN antibody
      • TFA antibody
      • Thromboplastin antibody
      • Tissue factor antibody
      see all

    Anti-Tissue Factor antibody [TF9-10H10] (ab35807)参考文献

    ab35807 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Sample Rhesus monkey Tissue sections (Aorta)
    Specification Aorta
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 03 2014

    Thank you for your inquiry. Please see the answer to your questions below: 1.) I can confirm that ab17375 (Anti-Tissue Factor antibody [2K1]) is derived from supernatant from hybridoma cells whereas ab35807 (Anti-Tissue Factor antibody ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"